Selected Protocols

Apparatus/equipment 

Sterile hood 

ClinoStar

Pipette boy and sterile disposable pipette 

(for 50 ml volume) 

Electronic or manual single channel pipette, 10–200 µl  

Sterile tips  

(for 10-200 µl volume)   

2 x sterile 10 ml syringes 

2 x sterile syringe needles 
(1.2 x 40mm, or 2.1 x 80mm recommended) 

Optical inverted microscope with camera and objectives (4x or 10x) 

Stopwatch 

Low adherent microwell plate 

10 ml Bioreactor 

Electronic or manual single channel pipette, 100–1000 µl  

Sterile tips  

(for 100-1000 µl volume)   

Sterile pipette tips, 10-200 µl volume, wide bore  

Automated Cell Counter (e. g.: Scepter, Millipore and tips for 60µl volume) 

Rack for 50 ml conical centrifuge tubes 

sterile 50 ml conical centrifuge tubes