Selected Protocols

Apparatus/equipment 

Sterile hood 

ClinoStar

Pipette boy and sterile disposable pipette 

(for 50 ml volume) 

Electronic or manual single channel pipette, 10200 µl  

Sterile tips  

(for 10-200 µl volume)   

2 x sterile 10 ml syringes 

2 x sterile syringe needles 
(1.2 x 40mm, or 2.1 x 80mm recommended) 

Optical inverted microscope with camera and objectives (4x or 10x) 

Stopwatch 

Low adherent microwell plate 

10 ml Bioreactor 

Electronic or manual single channel pipette, 1001000 µl  

Sterile tips  

(for 100-1000 µl volume)   

Sterile pipette tips, 10-200 µl volume, wide bore  

Automated Cell Counter (e. g.Scepter, Millipore and tips for 60µl volume) 

Rack for 50 ml conical centrifuge tubes 

sterile 50 ml conical centrifuge tubes 

With the ClinoStar system you also gain access to a library of protocols and application notes optimized for 3D cell cultivation with ClinoStar and downstream applications. Below is a list of some of our technical protocols you can assess by registration to our customer site. Check back to stay tuned as there are frequent updates to current and development of new protocols and applications notes using ClinoStar.

We have protocols within:

  • Media change 
  • Splitting spheroids 
  • ATP assay 
  • Protein determination 
  • Glucose determination 
  • Single cell suspension 
  • Q-spheroids 
  • And others…. 

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