How to work

Culturing spheroids and organoids is dependent on the cell model and sample being cultured. With the ClinoStar™ system we have created a generic system to generate spheroids and organoids in a reproducible fashion. The initial cell aggregation can be performed in several ways to accommodate various cell and sample types, but the downstream process remains. Each step breaks down the process of generating reproducible spheroids and organoids with ClinoStar™.

Spheroid and organoid development

Click on the steps to learn more

Spheroid and organoid development
Select aggregation method Transfer to ClinoReactor Cultivate in ClinoStar Mature spheroids

Select aggregation method

Select one of three aggregation methods

  • Low adherent microwell plate
  • Single cell suspension
  • Hydrogel droplets

We recommend using low adherent microwell plates for initial aggregation, to create the most uniform spheroids and organoids. If this is not possible due to the nature of the cell culture or sample behaviour, there are aggregation by self-assembly via single cell suspension or aggregation by hydrogel droplet formation.

Transfer to ClinoReactor

Initial spheroids and organoids or the single cell suspension are transferred to the 10 mL ClinoReactor.

In the ClinoStar system you can use your regular cell culture media and supplements. Once aggregated, you do not need to use additional scaffolds ECM growth factors or cytokines.

Cultivate in ClinoStar

Spheroids and organoids are cultivated to maturation in ClinoStar.

Cell culture media is routinely renewed, and irregular constructs are removed from the ClinoReactor to secure uniform growth.
Check that your spheroids or organoids are functional against a parameter that can be assayed in vivo. Primary cells may only require a few days in culture to recover this functionality. Cell lines usually need 2-3 weeks.

Mature spheroids

After end of the maturation period when the cells have recovered their functionality. For HEPG2/C3A cells there will be up to 350 hundrede spheroids in each ClinoReactor after a maturation period of 18 days.

The spheroids can be used for experiments from the period and for several weeks/months

Using this procedure and reguarly changing bioreactors the spheroids can be kept alive for up to 300 days.

You have your own farm of mature spheroids, providing an alternative to your animal model

Process of 3D Cell culture