Cell culture

It has been demonstrated over the past several decades that three-dimensional (3D) cell culture provides a superior system for mimicking the in vivo environment. Historically, flat, two-dimensional (2D) monolayer cells were the only practical option available, however, they are seriously limited in their ability to recapitulate normal tissue responses because they lack tissue-specific cytoarchitecture, in vivo mechanical and biochemical cues, and cell-to-cell interactions.

Spheroids and organoids have emerged as a more physiologically relevant model for in vivo cellular responses. These more complex models improve upon cell-line and primary cell-based methods with their ability to mimic native cytoarchitecture and to display physiological attributes of the native tissue. However, they can be difficult to culture for long periods of time, while maintaining human in vivo physiological performance. In addition, they often have size and shape variability, and poor viability.

 

Cell culture introduction

General introduction to cell culture and why is it used a a model, perhaps soem history and explanation of in vivo and in vivo

Suggested terms to include:

words to include: Tissue culture

what is cell culture used for, what are advantages of using a cell culture model, how to you create a cell culture, where does the clls come from, how do you grow them (what is media and serum)

 

 

 

    Figure text if needed and replace the image

    Statement or similar

    2D cell culture is good

    2D cell culture is bad

    2D cell culture

    explanation of 2D cell culture

    3D cell culture

    What si 3D cell culture 

    2D vs. 3D cell culture

    What is the advantage of using 3D over 2D and why when to use one over another. whay are 3D models better. disadvantages of 2D cell culture