Why use 3D?
In vivo, cells in tissues and tumors do not double every 2 – 4 days as they do in classical (2D) cell culture – so why use a technology developed in the 1950’s?
The CelVivo system creates an environment which promotes the growth and maintenance of large 3D tissue mimetic structures, whether they are spheroids, organoids, acini and other aggregates.
In this environment, liver spheroids remain in a stable dynamic equilibrium and exhibit human in vivo physiological performance for at least 24 days (i.e. up to at least 42 days of culture). During this time, the spheroids exhibit a stable transcriptome profile.
Reasons for Using 3D Cell Culture
Slows from a doubling time of 1 day to 60 days after 40 days in culture (see reference 3)
Resembles proliferation in the parental tissue or in tumours in vivo
Cultures can be maintained for over 300 days
Microfilaments (actin) microtubules (tubulin) and intermediate filaments (e.g. keratin) resemble that seen in vivo (see reference 7)
Resembles liver sections, showing plasma membrane differentiation, tight junctions, bile canaliculus-like channels, lipid droplets and glycogen granules (see reference 2)
Levels of ATP, urea and cholesterol reach levels seen in vivo (see reference 3)
Toxicity measurements for Amiodorone, APAP, Metformin, Phenformin and Valproic acid are more accurate than those obtained using human primary hepatocytes (see reference 1)
> 21 day old spheroids show clear aerobic metabolism (Warburg effect) (see reference 7)
Spheroids display histone marks and clipping (not seen using 2D cultures) (see reference 9)