Day -1:​

• HepG2/C3A cells were seeded into each of the wells of the low adherent microwell plate at a seeding density of 1.2 × 106, ​
• Centrifuged for 3 min at 120 x g​
• The cells were incubated in the low adherent microwell plate overnight to ensure aggregation​
• Consumables needed (link)​

Day 0:​

• Spheroids were detached from the microwell gently washing the wells with pre-warmed growth medium ​
• Detached spheroids quality was determined using a light microscope. ​
• Selected spheroids were then placed into bioreactors cultivated at 37 °C, 5% CO2, 95% air for 21 days ​
• Consumables needed (link)​