Vitamin K2 Modulates Vitamin D‐Induced Mechanical Properties of Human 3D Bone Spheroids In Vitrol

Maria Schröder, Elisabeth Aurstad Riksen,  Jianying He, Bjørn Helge Skallerud, Mona Elisabeth Møller, Aina‐Mari Lian, Unni Syversen, Janne Elin Reseland.
DOI: 10.1002/jbm4.10394 


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Characterization of an Alginate Encapsulated LS180 Spheroid Model for Anti-colorectal Cancer Compound Screening

Tanya Smit, Carlemi Calitz, Clarissa Willers , Hanna Svitina, Josias Hamman, Stephen J Fey, Chrisna Gouws, Krzysztof Wrzesinski

ACS Med Chem Lett, 2020, 11, 5, 1014-1021
DOI: 10.1021/acsmedchemlett.0c00076

Keywords: Cancer, Encapsulation, Antineoplastic agents, Cells, Biopolymers

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Response to and recovery from treatment in human liver-mimetic clinostat spheroids: a model for assessing repeated-dose drug toxicity

Stephen J Fey, Barbara Korzeniowska, Krzysztof Wrzesinski.
Toxicology Research, Volume 9, Issue 4, July 2020, Pages 379–389,
DOI: 10.1093/toxres/tfaa033 

Keywords: Drug toxicity, HepG2–C3A”>HepG2–C3A, recovery from treatment, repeated-dose, clinostat, 3D cell culture, acetaminophen, amiodarone, diclofenac, metformin, phenformin, valproic acid 

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A sub-chronic Xysmalobium undulatum hepatotoxicity investigation in HepG2/C3A spheroid cultures compared to an in vivo model

Carlemi Calitz, Josias H. Hamman, Stephen J. Fey, Alvaro M. Viljoen, Chrisna Gouws and Krzysztof Wrzesinski
Journal of Ethnopharmacology 239:111897. E-pub April 19, 2019
DOI: 10.1016/j.jep.2019.111897 

Keywords: Toxicology, HILI (Herb induced liver injury) DILI (Drug induced liver injury), correlation between rats and spheroids 

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Toxicity and anti-prolific properties of Xysmalobium undulatum water extract during short-term exposure to two-dimensional and three-dimensional spheroid cell cultures

Carlemi Calitz, Josias H. Hamman, Alvaro M. Viljoen, Stephen J. Fey, Chrisna Gouws and Krzysztof Wrzesinski
Toxicology Mechanisms and Methods 28-9, 641-652, 2018
DOI: 10.1080/15376516.2018.1485805

cell culturinghepatotoxicityherbal medicinespheroidthree-dimensional cell cultureUzaraUzarin

Keywords: Toxicology, HILI (Herb induced liver injury) DILI (Drug induced liver injury), correlation between 2D cell culture and spheroids

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Metabolic Reprogramming and the Recovery of Physiological Functionality in 3D Cultures in Micro-Bioreactors

Krzysztof Wrzesinski and Stephen J. Fey
Bioengineering, 5 (2) 1-25: 2018
DOI: 10.3390/bioengineering5010022

Keywords: Organoids; hypoxia; aerobic glycolysis; glutaminolysis; metabolic reprogramming; physiological performance; Warburg

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Recent advances in three-dimensional cell culturing to assess liver function and dysfunction: from a drug biotransformation and toxicity perspective

Carlemi Calitz, Josias H. Hamman, Stephen J. Fey, Krzysztof Wrzesinski & Chrisna Gouws
Toxicology Mechanisms and Methods, 2018
DOI: 10.1080/15376516.2017.1422580

Keywords: 3D modelsdrug biotransformationdrug toxicityin vitro modelsliver dysfunction

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Tissue-mimetic 3D cultures can determine the commercial potential of natural products and their derivatives

Krzysztof Wrzesinski and Stephen J. Fey
Biomed Biopharm Res. (14) 2 Supl. 271, 2017
DOI: 10.19277/bbr.14.2.168

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Cell-free DNA in a three-dimensional spheroid cell culture model: A preliminary study

Janine Aucamp, Carlemi Calitz, Abel J. Bronkhorst, Krzysztof Wrzesinski, Josias H Hamman, Chrisna Gouws and Pieter J. Pretorius
Int J Biochem Cell Biol. Aug; 89:182-192. Epub 2017 Jun 24, 2017
DOI: 10.1016/j.biocel.2017.06.014

Keywords: Cell-free DNA, 3D cell culture, Spheroid, Capillary electrophoresis

Rotating bioreactors

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Acetaminophen-induced S-nitrosylation and S-sulfenylation signalling in 3D cultured hepatocarcinoma cell spheroids

Katarzyna Wojdyla, Krzysztof Wrzesinski, James Williamson,  Stephen J. Fey, Adelina Rogowska-Wrzesinska
Toxicology Research 5(2) 905-920, 2016
DOI: 10.1039/C5TX00469A

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From 2D to 3D – a new dimension for modelling the effect of natural products on human tissue

Krzysztof Wrzesinski and Stephen J. Fey
Current Pharmaceutical Design 21(38): 5605-5616, 2015.
PMID: 26429710
DOI: 10.2174/1381612821666151002114227

Top-down and Middle-down Protein Analysis Reveals that Intact and Clipped Human Histones Differ in Post-translational Modification Patterns

Andrey Tvardovskiy, Krzysztof Wrzesinski, Simone Sidoli, Stephen J. Fey, Adelina Rogowska-Wrzesinska, Ole N. Jensen
Molecular and Cellular Proteomics 14(12):3142-53; 2015.
PMID: 26424599
DOI: 10.1074/mcp.M115.048975

Keywords: Natural products, 3D cell culture, metabolic equilibrium, spheroids, bioreactor, physiology, toxicology, efficacy.

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The cultural divide: exponential growth in classical 2D and metabolic equilibrium in 3D environments

Krzysztof Wrzesinski, Adelina Rogowska-Wrzesinska, Rattiyaporn Kanlaya, Kamil Borkowski, Veit Schwämmle, Jie Daia, Kira E. Joensen, Katarzyne Wojdyla, Vasco Botelho Carvalho & Stephen J. Fey
PLOS One 9(9) 1-15; 2014
PMID: 25222612
DOI: 10.1371/journal.pone.0106973

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Heteromer score – using internal standards to assess the quality of proteomic data

Adelina Rogowska-Wrzesinska, Krzysztof Wrzesinski and Stephen J. Fey
Proteomics. 14(9):1042-7 2014
PMID: 24616253
DOI: 10.1002/pmic.201300457

Keywords: Bioinformatics, Cell biology, Heterodimer, Heteromer score, Internal standard, Quality control

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Microgravity spheroids as a reliable, long term tool for predictive toxicology

Stephen J. Fey and Krzysztof Wrzesinski
Toxicology Letters, 221S S153 2013.
DOI: 10.1016/j.toxlet.2013.05.318

Determination of acute lethal and chronic lethal dose thresholds of Valproic acid using 3D spheroids constructed from the immortal human hepatocyte cell line HepG2/C3A

Stephen J. Fey and Krzysztof Wrzesinski
In ”Valproic Acid: Pharmacology, Mechanisms of Action and Clinical Implications” Nova Science Publishers, New York. Ch. V, 141-165; 2013

Human liver spheroids exhibit stable physiological functionality for at least 24 days after recovering from trypsinisation

Krzystof Wrzesinski, Maria C. Magnone, Line Visby Hansen, Marianne Ehrhorn Kruse, Tobias Begauer, Maria Bobadilla, Marcel Gubler, Jacques Mizrahi, Christina Møller Andreasen, Kelan Zhang, Kira Eyed Joensen, Signe M. Andersen, Jacob Bastholm Olesen, Ove B. Schaffalitzky de Muckadel and Stephen J. Fey
Toxicology Research; 2(3) 163-172; 2013
DOI: 10.1039/C3TX20086H
NB: Manuscript featured on the front cover of the journal.

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After trypsinisation, 3D spheroids of C3A hepatocytes need 18 days to re-establish similar levels of key physiological functions to those seen in the liver

Krzystof Wrzesinski and Stephen J. Fey
Toxicology Research; 2(2) 123-135; 2013
DOI: 10.1039/C2TX20060K
NB: Manuscript featured on the front cover of the journal.

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Determination of drug toxicity using 3D spheroids constructed from immortalized human hepatocytes

Stephen J. Fey and Krzysztof Wrzesinski
Toxicological Sciences 127(2) 403-411; 2012.
PMID: 22454432
DOI: 10.1093/toxsci/kfs122

Keywords: spheroid culture, drug toxicity, planimetry, protein-to-drug ratio, in vivo/in vitro correlation, LD50/LC50

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